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Sample accessioning Genomics core · Cambridge · todaysynthetic
| Accession | Type | Donor | In | Arrived | Consent | Status | Location |
|---|---|---|---|---|---|---|---|
| LS26-041872 | Urine cfDNA | P-0417-031 | 11:52 | 4 °C | v3.2 | Accessioned | F2 · S1 R1 B1 |
| LS26-041871 | Plasma | P-0388-038 | 11:43 | 4 °C | v3.2 | Accessioned | F2 · S2 R2 B2 |
| LS26-041870 | Whole blood EDTA | P-0417-045 | 11:34 | 4 °C | v3.2 | In prep | F2 · S3 R3 B3 |
| LS26-041869 | Fresh tumour | P-0398-052 | 11:25 | dry ice | v3.2 | Accessioned | F2 · S4 R4 B4 |
| LS26-041868 | FFPE curls | P-0412-059 | 11:16 | RT | v3.2 | Haemolysed | F2 · S5 R1 B5 |
| LS26-041867 | Whole blood EDTA | P-0417-066 | 11:07 | 4 °C | v3.2 | Accessioned | F2 · S1 R2 B6 |
| LS26-041866 | Fresh tumour | P-0398-073 | 10:58 | dry ice | v3.2 | Accessioned | F2 · S2 R3 B7 |
| LS26-041865 | FFPE curls | P-0412-080 | 10:49 | RT | v3.2 | Accessioned | F2 · S3 R4 B8 |
| LS26-041864 | Urine cfDNA | P-0417-087 | 10:40 | 4 °C | v3.2 | Accessioned | F2 · S4 R1 B9 |
| LS26-041863 | Plasma | P-0388-094 | 10:31 | 4 °C | Check | Label mismatch | F2 · S5 R2 B1 |
| LS26-041862 | FFPE curls | P-0412-101 | 10:22 | RT | v3.2 | Accessioned | F2 · S1 R3 B2 |
| LS26-041861 | Urine cfDNA | P-0417-108 | 10:13 | 4 °C | v3.2 | Accessioned | F2 · S2 R4 B3 |
| LS26-041860 | Plasma | P-0388-115 | 10:04 | 4 °C | v3.2 | Accessioned | F2 · S3 R1 B4 |
| LS26-041859 | Whole blood EDTA | P-0417-122 | 09:55 | 4 °C | v3.2 | In prep | F2 · S4 R2 B5 |
| LS26-041858 | Fresh tumour | P-0398-129 | 09:46 | dry ice | v3.2 | Accessioned | F2 · S5 R3 B6 |
| LS26-041857 | Plasma | P-0388-136 | 09:37 | 4 °C | v3.2 | Accessioned | F2 · S1 R4 B7 |
| LS26-041856 | Whole blood EDTA | P-0417-143 | 09:28 | 4 °C | v3.2 | Accessioned | F2 · S2 R1 B8 |
| LS26-041855 | Fresh tumour | P-0398-150 | 09:19 | dry ice | v3.2 | Temp excursion | F2 · S3 R2 B9 |
| LS26-041854 | FFPE curls | P-0412-157 | 09:10 | RT | v3.2 | Accessioned | F2 · S4 R3 B1 |
| LS26-041853 | Urine cfDNA | P-0417-164 | 09:01 | 4 °C | v3.2 | Accessioned | F2 · S5 R4 B2 |
| LS26-041852 | Fresh tumour | P-0398-171 | 08:52 | dry ice | v3.2 | Accessioned | F2 · S1 R1 B3 |
| LS26-041851 | FFPE curls | P-0412-178 | 08:43 | RT | v3.2 | In prep | F2 · S2 R2 B4 |
| LS26-041850 | Urine cfDNA | P-0417-185 | 08:34 | 4 °C | v3.2 | Accessioned | F2 · S3 R3 B5 |
| LS26-041849 | Plasma | P-0388-192 | 08:25 | 4 °C | v3.2 | Accessioned | F2 · S4 R4 B6 |
| LS26-041848 | Whole blood EDTA | P-0417-199 | 08:16 | 4 °C | v3.2 | Accessioned | F2 · S5 R1 B7 |
| LS26-041847 | Urine cfDNA | P-0417-206 | 08:07 | 4 °C | v3.2 | Accessioned | F2 · S1 R2 B8 |
| LS26-041846 | Plasma | P-0388-213 | 07:58 | 4 °C | v3.2 | Accessioned | F2 · S2 R3 B9 |
| LS26-041845 | Whole blood EDTA | P-0417-220 | 07:49 | 4 °C | v3.2 | Accessioned | F2 · S3 R4 B1 |
| LS26-041844 | Fresh tumour | P-0398-227 | 07:40 | dry ice | v3.2 | Accessioned | F2 · S4 R1 B2 |
| LS26-041843 | FFPE curls | P-0412-234 | 07:31 | RT | v3.2 | Accessioned | F2 · S5 R2 B3 |
| LS26-041842 | Whole blood EDTA | P-0417-241 | 07:22 | 4 °C | v3.2 | Accessioned | F2 · S1 R3 B4 |
| LS26-041841 | Fresh tumour | P-0398-248 | 07:13 | dry ice | v3.2 | Accessioned | F2 · S2 R4 B5 |
Plate PL-261012-01 KAPA HyperPrep · ng/µl after ligation · 84 samples, 2 PC, 2 NTC2 low yield
Library prep batch LB-2026-0412Step 5 of 7
| # | Library prep step | By | At | State | QC |
|---|---|---|---|---|---|
| 1 | Fragmentation | S. Baird | 08:40 | Done | 200 bp median |
| 2 | End repair, A-tail | S. Baird | 09:15 | Done | - |
| 3 | Adapter ligation | S. Baird | 09:50 | Done | UDI set B |
| 4 | Clean-up 0.8× | Hamilton STAR | 10:35 | Done | - |
| 5 | PCR 8 cycles | Hamilton STAR | 11:05 | Running | est. 11:52 |
| 6 | Hybrid capture | - | 13:00 | Booked | Twist 1.2 Mb |
| 7 | QC Qubit + TapeStation | - | Tue | Booked | pass ≥ 2 nM |
Freezer map Genomics core · 4 freezers · 2,912 boxesF2 normal
LS26-041872 chain of custodyUnbroken
SampleUrine cfDNA · 8.0 mlDonorP-0417-031 · Desmond PryceNHS number999 000 0631StudyPRJ-0417 · REC 24/NW/0187Consentv3.2 · genomic + future useAliquotsA4 in prep · A5 stored
Custody8 signed steps
| When | Event | By |
|---|---|---|
| Fri 16:05 | Collected, urology clinic | R. Lowe |
| Fri 16:40 | Sealed, bag TB-88214 | R. Lowe |
| Sat 08:10 | Courier, 4 °C logger on | Medilink |
| Sat 10:52 | Received, seal intact | S. Baird |
| Sat 11:03 | Accessioned, barcode printed | S. Baird |
| Sat 11:40 | cfDNA extracted, 2 × 40 µl | STAR |
| Sat 12:05 | Stored F2 · S1 R1 B1 · A4 | S. Baird |
| 08:40 | Out for library prep | S. Baird |
Courier temperature2 to 8 °C held
Aliquots3
| Aliquot | Volume | Where |
|---|---|---|
| A4 cfDNA | 40 µl | Plate PL-261012-01 A1 |
| A5 cfDNA | 40 µl | F2 · S1 R1 B1 · A5 |
| A6 urine pellet | 1.5 ml | F2 · S1 R1 B1 · A6 |
Next decision
ReleaseA4 library to run 241014HoldLS26-041863 label mismatch
Release A4Resolve mismatch
Each transfer signed with EDGE ID · hash chained
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