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Per-sample reads run 241012_LH00418_0117_B22T7KLT3 · 24 samples · Twist custom 1.2 Mbsynthetic
| # | Sample | Project | i7 - i5 | Reads M | Q30 % | Dup % | Mean cov | QC |
|---|---|---|---|---|---|---|---|---|
| 1 | BLD-0417-289 | PRJ-0417 | CGCTCATT-AGGCTATA | 55.2 | 95.0 | 11.7 | 933× | Pass |
| 2 | BLD-0417-290 | PRJ-0417 | GAGATTCC-AGGCTATA | 53.0 | 94.4 | 19.7 | 913× | Pass |
| 3 | BLD-0417-291 | PRJ-0417 | ATTCAGAA-AGGCTATA | 48.9 | 94.0 | 18.6 | 829× | Pass |
| 4 | BLD-0417-292 | PRJ-0417 | GAATTCGT-AGGCTATA | 45.8 | 86.9 | 20.4 | 840× | Warn: Q30 |
| 5 | BLD-0417-293 | PRJ-0417 | CTGAAGCT-AGGCTATA | 55.1 | 93.9 | 16.8 | 946× | Pass |
| 6 | BLD-0417-294 | PRJ-0417 | TAATGCGC-AGGCTATA | 38.6 | 92.5 | 17.0 | 662× | Pass |
| 7 | BLD-0417-295 | PRJ-0417 | CGGCTATG-AGGCTATA | 8.2 | 94.8 | 41.8 | 141× | Fail: yield |
| 8 | BLD-0417-296 | PRJ-0417 | TCCGCGAA-AGGCTATA | 42.3 | 92.5 | 15.2 | 710× | Pass |
| 9 | BLD-0417-297 | PRJ-0417 | TCTCGCGC-AGGCTATA | 47.2 | 95.1 | 19.8 | 800× | Pass |
| 10 | BLD-0417-298 | PRJ-0417 | AGCGATAG-AGGCTATA | 54.1 | 94.6 | 20.5 | 979× | Pass |
| 11 | BLD-0417-299 | PRJ-0417 | ATTACTCG-AGGCTATA | 51.7 | 94.5 | 15.6 | 944× | Pass |
| 12 | BLD-0417-300 | PRJ-0417 | TCCGGAGA-AGGCTATA | 55.3 | 92.8 | 20.8 | 985× | Pass |
| 13 | BLD-0417-301 | PRJ-0417 | CGCTCATT-GCCTCTAT | 46.3 | 93.8 | 17.4 | 844× | Pass |
| 14 | BLD-0417-302 | PRJ-0417 | GAGATTCC-GCCTCTAT | 47.0 | 88.1 | 15.6 | 853× | Warn: Q30 |
| 15 | BLD-0417-303 | PRJ-0417 | ATTCAGAA-GCCTCTAT | 54.2 | 93.6 | 18.4 | 982× | Pass |
| 16 | BLD-0417-304 | PRJ-0417 | GAATTCGT-GCCTCTAT | 51.0 | 93.7 | 13.9 | 879× | Pass |
| 17 | CFM-0388-057 | PRJ-0388 | CTGAAGCT-GCCTCTAT | 50.6 | 92.8 | 22.8 | 867× | Pass |
| 18 | CFM-0388-058 | PRJ-0388 | TAATGCGC-GCCTCTAT | 54.4 | 93.2 | 23.4 | 969× | Pass |
| 19 | CFM-0388-059 | PRJ-0388 | CGGCTATG-GCCTCTAT | 47.1 | 93.8 | 19.5 | 831× | Pass |
| 20 | CFM-0388-060 | PRJ-0388 | TCCGCGAA-GCCTCTAT | 11.4 | 93.0 | 17.7 | 203× | Fail: yield |
| 21 | CFM-0388-061 | PRJ-0388 | TCTCGCGC-GCCTCTAT | 49.2 | 92.6 | 21.7 | 879× | Pass |
| 22 | CFM-0388-062 | PRJ-0388 | AGCGATAG-GCCTCTAT | 54.3 | 92.9 | 33.4 | 916× | Warn: dup |
| 23 | CFM-0388-063 | PRJ-0388 | ATTACTCG-GCCTCTAT | 49.8 | 93.1 | 13.9 | 901× | Pass |
| 24 | CFM-0388-064 | PRJ-0388 | TCCGGAGA-GCCTCTAT | 39.9 | 93.8 | 22.1 | 678× | Pass |
Undetermined barcodes 19.1 M reads · top 4 index pairsNo swap found
| i7 - i5 | Reads M | Nearest sample | Cause |
|---|---|---|---|
| CGGCTATG-AGGCTAGA | 4.2 | BLD-0417-295 | 1 mismatch in i5 |
| GGGGGGGG-AGATCTCG | 3.7 | none | Dark cycle, poly-G |
| TCCGCGAA-GCCTCTAT | 1.9 | CFM-0388-060 | Index hopping 0.3% |
| AGCGATAG-AGGCTAGA | 1.1 | BLD-0417-298 | 1 mismatch in i5 |
Run QC NovaSeq X Plus LH00418 · 10B flow cell · 2×151Lane 7 below spec
Q30 by cycle322 cycles · 8 lanes
Coveragefraction of targets at or above depth
Adapter contenttrimmed by fastp 0.23.4
Run metrics B22T7KLT3Analysing
InstrumentNovaSeq X Plus · LH00418SiteGenomics core · CambridgeChemistryXLEAP-SBS · 300 cycle kitRead plan151 + 10 + 10 + 151Yield3.04 Tb of 3.0 Tb specClusters PF10.05 B · 82.4% PF% ≥ Q3093.4% · spec 85%Error rate0.38% (PhiX 1%)Occupied97.1%Undetermined1.9% · 19.1 M readsStartedSat 11 Oct 06:12 · 25h 48m
Lanes1.2 Gb per lane
| Lane | PF M | Q30 | Err % | QC |
|---|---|---|---|---|
| 1 | 1,268 | 94.1 | 0.34 | Pass |
| 2 | 1,271 | 94.3 | 0.33 | Pass |
| 3 | 1,259 | 93.8 | 0.36 | Pass |
| 4 | 1,266 | 94.0 | 0.35 | Pass |
| 5 | 1,270 | 94.2 | 0.34 | Pass |
| 6 | 1,262 | 93.9 | 0.36 | Pass |
| 7 | 1,188 | 89.6 | 0.61 | Warn |
| 8 | 1,265 | 94.0 | 0.35 | Pass |
Next decision
Release22 samples to sarekRequeue-007, -020 on run 241014Lane 7keep, Q30 89.6% above floor
Release 22Requeue 2Hold run
QC lead signs · receipt to audit chain
Timeline12 Oct
| Sat 06:12 Run started, PhiX 1% spike |
| Sun 07:40 Sequencing complete, 25h 28m |
| 08:14 bcl-convert demultiplexed 24 |
| 09:02 Jude flags -007, -020 and lane 7 |
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